Alternative to the Pyramid Scheme of “Aspirational content”

Youtube put out a caution about burnout. Penny arcade summed it up. “Creators: you’re feeling burned out because you are light bulbs. And there are always more light bulbs. You create young, energetic light bulbs with every video you make.”

Good educational content is distinct from aspirational content. As a chemistry professor, my primary job is to produce more chemists (and educate non-chemists who need the background). The point is not to generate a great many more chemistry professors. Complexly and the Scishow folks make great educational content. Aspirational content is the opposite: it endeavors to sell the idea that you too can become an aspirational content producer. Mostly by watching more aspirational content.

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Build video for the iron battery

I made a youtube video every weekday in 2017. That experience pulled me into the world of battery chemistry. Daily vlogging was hard but taught me something about social media, science outreach, and the topics that people are interested in. I was a little surprised to find that people were so interested in batteries. I like the opportunity to explore something that is of wide interest. Everyone has a battery in their pocket, and everyone wants them to last longer.

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qPCR and graphene for aptamer selection

We’ve been selecting aptamers in the lab for the last year. Having a qPCR on the bench has really helped, and so we wrote up a methods paper in ACS Combinatorial Science. The company that made our qPCR instrument has put up a blurb about it, too.

The qPCR function is great for cycle course optimization, and we have been using the melt curve analysis function of the Open qPCR (thermofluorimetry) to do a binding assay. It works pretty well. We put a dye in with the aptamer and measure the temperature at which the dye dye-DNA complex melts. The bound aptamer has a different melt temperature, so it gives a specific signal. We plot that specific signal as a function of concentration and to determine the binding constant. It’s based on the Easley lab’s method paper from 2015 with low-cost equipment.

The instrument simplifies some of the more touchy parts of the aptamer selection. Undergrads have been turning rounds pretty efficiently this year with the help of the open qPCR instrument.

We have also been using graphene oxide to try some selections. I have only heard of graphene oxide SELEX recently, but it grabs unstructured DNA to separate them from aptamers bound to target. It’s looking good. I hope to report on that soon.


Thank you to Crowdfunding Supporters

Thank you to all of the kind supporters who helped raise money for undergraduate research in iron batteries here at the U of Idaho. Together we put together $5000 that will be put toward a fellowship and materials for a student to explore this and we will put together a open source plans document next year. We’re also going to document the process with a weekly video about the project, so please do stay tuned.