Tag Archives: chemistry

Raspberry Pi Computer: standalone “safe” machine

I want a computer that does not rely on a software service agreement to function. Cell phones obviously have to operate as a service since they need a network to operate. The phone is a gateway to the cell service. My kindle is similar for Amazon services. Laptops feel different to me. Computers feel like products. I own my laptop and I want to think of it as a standalone device, not a gateway to a cloud service.

Windows 10 is now a service. The future is clearly going in the direction of software as a service (SaaS as the kids put it). That’s fine, but I like to have at least some device that can’t be remotely bricked by a company.

Raspberry Pi Computer in a Box parts list:

Quick catch-up for other topics this week: I made a video I about sodium-ion batteries and people seemed interested. I think a sodium ion battery would be really cool. But I think the expectations of the youtube viewers may be a little inflated. Sodium is heavier than lithium. It yields less energy per atom, too. So it’s not going to be great for mobile. I made another video talking about that. So why bother with sodium? Lithium is relatively rare and expensive… so sodium might be better for stationary applications. It’s hard to say at this point, but I’m investing my time in an iron battery.

 

Index of refraction based detection

I had an idea to build an index of refraction-based detector for an electrophoresis set-up. When light passes through a prism, it bends. That’s how prisms cast rainbows. The angle at which the rainbow shows up depends on the material of the prism. If the prism is made of glass, you get a rainbow at one angle. If the prism is made of water, you get a different angle. We can use this phenomenon to detect changes in the material. We need a prism that we can fill with different materials. Then we can see how the angle changes. I built such a prism out of acrylic with my laser cutter. It looks like this:

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I haven’t started flowing things through the prism yet. I’m still figuring out how to detect the angle change. But there definitely is an angle change. I can see the difference in angle between when I have air in the prism and when I have water in the prism. Have a look – that’s air on the left and water on the right:

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So now the question is how tiny a difference can I detect? Adding small amounts of sugar to the water will change the index of refraction a tiny bit. That should change the angle by a small amount as well. If I can measure the angle very precisely, I should be able to detect very small changes in the sugar content of the water.

I’ll try to get my students to build generation 2 of the device. Design, build, test learn. The work will teach them.

More droplet literature reading and a bit of a ramble

There is a neat paper in this issue of the journal Lab on a Chip. The Tuteja lab out of Michigan developed a clever way of making droplets using a laser cut jig and an open platform. I mean literally open (not like open source). They make water in oil droplets that float across the surface of a hydrophobic chip. It reminds me a lot of the beer sphere.

I caught this image of a beer sphere suspended on its surface tension on the surface of a glass of beer. It was so persistent that I was able to get my camera and come back to the table to take a picture.

The Slo-Mo guys got really good footage of surface tension droplets. It’s an interesting phenomenon. Surface tension prevents a water droplet from merging with the water surface. If you have an oil surface, the water will not merge at all. Surface tension isn’t needed. But it looks similar.

I meant to get in the lab this morning and fabricate some devices, but I am not feeling motivated. I ramble a bit about that in the video today. I’m very worried about science funding. That’s dumb. Can’t do anything about it. It’s worrying for nothing. But I am worrying anyway. That’s four hours of my life I will not get back.

Focus on science. Rise above.

 

Papers on droplet microfluidics I am reading

Monodispersed microfluidic droplet generation by shear focusing microfluidic device,” is from 2006. It’s a study on the design of the flow focusing droplet generator. It explores the role of flow rate and pinched geometry on the droplets. At the time, it wasn’t completely clear what these droplets could be used for. I was looking at them as little storage containers for cells.

In 2016, digital PCR was a clear application of this technology. A PCR reaction was segregated into lots of little droplets. Each droplet either has a DNA molecule or does not. As a consequence, the PCR reaction makes it go green or not. Instead of trying to interpret different levels of green fluorescence (which is relatively hard to quantify), the scientist can just count the bright droplets (much easier to quantify). “Centrifugal micro-channel array droplet generation for highly parallel digital PCR” presents an unconventional droplet generator to make lots of little droplets for that application.

The application I’m working toward is a little different. I want to make particles based on these droplets. The little particles will have a sensor on them so that we can detect what is happening near to the particle. The particle might then respond by glowing green or by releasing a drug. Similar particles have applications in cosmetics and lubricants. I think that we can make them smarter. We can apply them to research (reporting cell environments) diagnostics and maybe therapeutics (some day).

Acrylic microfluidics part 7

One of the reasons I am trying to develop these techniques for acrylic microfluidics is to shorten the design-build-test feedback loop. With the PDMS techniques I worked on and grad school the loop is about a week long. It takes a day to design, a few days to get the photomask, and a day or two to fabricate. If nothing goes wrong, I use to be able to get a design tested on day five.

With acrylic based microfluidics, the loop is much shorter. I can modify a design, cut it with the laser, fabricate the chip, and have it tested all within three hours. Three hours is a significant improvement over five days.

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This morning I came in and cut a new chip with some new parameters. I was hoping to see a narrower width channel which would produce smaller droplets. I didn’t get that, but I can try again (tomorrow not next week). Plus, I learned something. I need to add a viewing area to the chip to see the droplets better.

I made a little video of some of the process. I skipped the bonding step in the video because it’s just 10 minutes of waiting while the hot press does its work.

Here are parts 1 2 3 4, 5 and 6.